Generator

Part:BBa_M36363:Design

Designed by: Will Connors, Ethan Li, and Shirbi Ish-Shalom   Group: Stanford BIOE44 - S11   (2013-10-25)


PoPS -> DbjA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 468
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 468
    Illegal NheI site found at 40
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 468
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 468
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 468
    Illegal NgoMIV site found at 984
    Illegal AgeI site found at 713
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 774


Design Notes

The 5' BCD UTR was not originally intended to be weak, but an error in selection led to the use of the incorrect BCD part.


Source

DbjA haloalkane dehalogenase is from the Bradyrhizobium japonicum USDA110 genome. The 5' BCD UTR and terminator are both from the BIOFAB database.

References